Collection Tubes

Collection Tubes for Blood Specimens

| Gold/Red-Gray Marble | Mint Green | Green | Lavender | Light Blue |

| Gray | Red | Royal Blue | Yellow |

The following tables should be helpful in understanding which kind of vacuum (VACUTAINER®) type tube to use in the collection of specimens. For manufacturers guidelines see BD Vacutainer Order of Draw for Multiple Tube Collections. Refer to the Test Table for specific instructions and sample requirements.

Gold or Red-Gray Marble Stopper (two-types) - SST, Serum Separator Tube
Additive:  Polymer gel and powdered glass clot activator
Stopper Type: HemogardTM Gold top/Conventional Red-Gray Marble 
Tube Type/Size: Plastic Tube 13 x 100/16 x 100 
Specimen Type:  Serum 
Draw Amount:   5.0 mL/8.5 mL 
Inversions: 
Laboratory Use:   Sterile SST® brand tube with inert clot activator determinations that require serum in chemistry or infectious disease testing. Gel separates serum from cells. Tube inversion ensures mixing of clot activator with blood and clotting within 30 minutes.
 

Mint Green Stopper - PST, Plasma Separator Tube: HOSPITAL PATIENTS ONLY
Additive: Lithium Heparin, Polymer gel plasma separator 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic tube 13 x 75 
Specimen Type: Whole Blood, Plasma 
Draw Amount: 3.0 mL
Inversions:
Laboratory Use: General chemistries and some therapeutic dogs. DO NOT USE FOR LITHIUM TESTING. 

Green Stopper Tube (two types): Dark Green or Clear Green
Additive: Sodium Heparin 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic tube 13 x 75 
Specimen Type:  Whole Blood, Plasma 
Draw Amount: 4.0 mL/2.0 mL (pediatric tube) 
Inversions:
Laboratory Use:  For plasma determinations in chemistry. Tube inversion prevents clotting. Dark green 4.0 mL Sodium Herparin used for chromosome studies.  

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Lavender Stopper Tube (two types): Dark Lavender or Clear Lavender
Additive: EDTA-K2 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic Tube 13 x 75 
Specimen Type: Whole Blood, Plasma 
Draw Amount: 4.0mL/2.0mL (pediatric tube) 
Inversions:
Laboratory Use:  Dark Lavender 4.0 mL for whole hematology, ammonia, lead, HIV RNA, HbA1C quantitation determinations and for blood bank testing. Tube inversion prevents clotting.  

 Light Blue Stopper Tube (two types): Solid Light Blue or Clear Light Blue
Additive: Sodium Citrate (3.2%, 0.109M) 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic tube 13 x 75 
Specimen Type: Whole Blood, Plasma 
Draw Amount: 2.7 mL/1.8 mL (pediatric tube only) 
Inversions: 4 (gently) 
Laboratory Use: For coagulation determinations of plasma specimens. Tube inversion prevents clotting. 
Note: Certain tests require chilled specimens. Follow recommended procedures for collection, temperature and transporting of coagulation specimens.  

 Gray Stopper Tube:
Additive: Sodium fluoride/Potassium oxalate 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic 13 x 75 
Specimen Type: Whole Blood, Plasma 
Draw Amount: 4.0 mL 
Inversions:
Laboratory Use: For glucose, toxicology determinations. Antiglycolytic additives stabilize glucose values for up to 24 hours at room temperature. Tubes should be filled to 4.0 mL. Tube inversion ensures proper mixing of additive and blood.  

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Red Stopper Tube:
Additive:
 Inert Clot Activator (powdered gas)
Stopper Type:  HemogardTM
Tube Type/Size: Plastic 13 x 75 
Specimen Type:  Serum
Draw Amount: 6.0 mL 
Inversions:  8
Laboratory Use:  For serum determinations in chemistry, serology and blood bank testing. Can be used as sterile transport tube. 

 Royal Blue Stopper Tube: (two types) No additive or EDTA
Additive: None/EDTA 
Stopper Type: HemogardTM 
Tube Type/Size: Plastic 13 x 100 
Specimen Type: Whole Blood, Plasma 
Draw Amount: 6.0 mL 
Inversions:
Laboratory Use:  For trace element, toxicology and nutrition determinations. Special stopper formulation offers the lowest verified levels of trace elements available. Refer to specific test for proper tube.  

 Yellow Stopper Tube: (two types) Solution A or Solution B
Additive: ACD, Solution A 
Stopper Type: Conventional 
Tube Type/Size: Glass 16 x 100 
Specimen Type:  Whole blood 
Draw Amount:  8.5 mL 
Inversions: 
Laboratory Use:  Tissue typing and some Red Cross testing. Refer to specific test for proper tube. 
   
Additive: ACD, Solution B 
Stopper Type:  Conventional 
Tube Type/Size:  Glass 16 x 100 
Specimen Type:  Whole blood 
Draw Amount:  6.0 mL 
Inversions: 
Laboratory Use:  Tissue typing. Refer to specific test for proper tube.  

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