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BCR-ABL1, QUALITATIVE WITH REFLEX TO BCR-ABL1 QUANTITATIVE

Mnemonic

BCR RLFX

Guidelines

For use in detecting the presence of a BCR-ABL1 fusion, for determining breakpoint status, and for baseline quantitation. For ordering guidelines see ARUP.

Specimen Collection Requirements

Collect

Whole Blood, lavender (EDTA) top tube or Bone Marrow in EDTA

Handling

Specimens must be received at ARUP within 48 hours of collection due to lability of RNA.

Preferred Volume

5.0 mL Whole Blood or 3.0 mL Bone Marrow

Minimum Volume

1.0 mL Whole Blood or 1.0 mL Bone Marrow

Transport

Refrigerated

Rejection Criteria

Serum or plasma, specimens collected in preservatives other than EDTA, severely hemolyzed, frozen or clotted whole blood or bone marrow

Stability

Ambient: 1 hour; Refrigerated: 48 hours; Frozen: Unacceptable

Testing Information

Note

This reflex assay is recommended when the BCR-ABL1 fusion form is not known or unclear. This reflex assay detects the presence of either the p210 (major breakpoint) or p190 (minor breakpoint). If the presence of either the p210 or p190 BCR-ABL1 fusion is detected, then the appropriate quantitative test will be performed.

If the fusion form is known, refer to BCR-ABL1, Major (p210), Quantitative or BCR-ABL1, Minor (p190), Quantitative.

Performed

Sun, Tue - Fri

Reported

7-10 days

Method

Reverse Transcription Polymerase Chain Reaction

Referral Lab

ARUP (2005010)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

81206, 81207, G0452, if reflexed, add 81206 or 81207

Last Updated: Monday, September 25, 2017 11:23:09 AM


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