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BILE ACIDS, FRACTIONATED AND TOTAL

Mnemonic

BILE FRACT

Guidelines

Do not use to diagnose liver dysfunction. Use to screen for inborn errors of bile acid metabolism or monitor bile acid therapy.

Specimen Collection Requirements

Patient Preparation

Patient should fast for a minimum of eight hours prior to specimen collection

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

Handling

Allow serum to clot completely at room temperature (minimum: SST – 30 minutes and red top: 60 minutes). Centrifuge and separate serum from cells. Transfer 1 mL serum to an aliquot tube. 

Preferred Volume

1.0 mL Serum

Minimum Volume

0.2 mL Serum (whole blood 0.6 mL minimum draw)

Transport

Refrigerated (2-8 ºC)

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 2 weeks; Frozen (≤ -20°C): 3 months

Testing Information

Performed

Tue, Thu, Sat

Reported

3-8 days

Method

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Referral Lab

ARUP (92610)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

83789

Last Updated: Friday, January 5, 2018 06:58:48 AM


Client Services: 503-413-1234