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BRAF V600 MUTATION ANALYSIS BY PCR

Synonym

BRAF - V600E mutation - BRAF RGQ PCR

Includes

The BRAF RGQ PCR Kit enables the detection of 5 somatic mutations at codon 600 of the BRAF oncogene against a background of wild type genomic DNA.

Specimen Collection Requirements

Collect

Formalin fix (10 percent neutral buffered formalin) and paraffin embedded tissue. Protect from excessive heat. Transport tissue block or 5 unstained 5-10micron slides. Minimum 3 slides.

Preferred Volume

FFPE Block or 5 unstained 5-10micron slides

Minimum Volume

3 unstained 5-10micron slides

Transport

Room Temperature

Rejection Criteria

Specimens fixed/processed in alternative fixatives (alcohol, Prefer®), fresh, frozen or decalcified specimens

Stability

Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable

Testing Information

Performed

Wed-Thu

Method

This is a real time PCR assay using the Qiagen BRAF RGQ PCR kit to detect 5 mutations in codon 600 of the BRAF oncogene against a background of wild type genomic DNA.

Reference Values

Reference Values

No mutation.

CPT Codes

81210

Last Updated: Thursday, January 5, 2017 03:40:26 AM


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