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* CALCIUM, IONIZED, PLASMA AND WHOLE BLOOD

Mnemonic

CA ION P

Synonym

Ionized Calcium Plasma and WB - Calcium Ionized Level - Calcium Free - Calcium - iCa

Guidelines

To be used for Inpatient, ED and/or surgical testing.

Specimen Collection Requirements

Collect

PREFFERED: Whole Blood, one 3 mL green (lithium heparin, PST) top tube or one lithium heparin blood gas syringe, also acceptable: Plasma, one 3 mL mint green (Lithium heparin, PST) top tube

Handling

All specimens should be collected under anaerobic conditions. For anaerobic samples, do not remove the top of the tube before analysis. Any loss of CO2 will increase the pH, which decreases ionized calcium. Do not store specimens on ice. LITHIUM HEPARIN WHOLE BLOOD: Send unspun. Whole blood should be analyzed within 30 minutes of collection. Do not remove top from tube. LITHIUM HEPARIN PLASMA: Spin PST within 1 hour of collection. Do not remove top from tube.

Preferred Volume

1.0 mL whole blood or plasma

Minimum Volume

0.5 mL whole blood or plasma (1.2 mL minimum whole blood draw, if testing plasma) 

Transport

Room temperature

Rejection Criteria

Severely hemolyzed specimens. Sodium heparin, EDTA, citrate, oxalate and sodium fluoride specimens.

Stability

WHOLE BLOOD: Room Temperature (18-26°C): 30 minutes; Refrigerated (2-8°C): Unacceptable; Frozen (≤ -20°C): Unacceptable

PLASMA: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 48 hours; Frozen (≤ -20°C): 72 hours

Testing Information

Performed

7 days/week

Reported

30 minutes

Method

Ion selective electrode.

Reference Values

Reference Values

Critical Values

For critical age related ranges see Chemistry Age Related Reference Ranges and Critical Values.

CPT Codes

82330

Last Updated: Thursday, March 2, 2017 01:41:21 PM


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