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DIRECT ANTIGLOBULIN TEST

Mnemonic

BB DAT

Synonym

Direct Coombs' - DAT

Includes

Direct Antiglobulin Test with polyspecific anti-human globulin reagent. It may include use of monospecific reagents (anti-IgG, anti-complement) when indicated.

Guidelines

For detection of immunoproteins coating red cells (IgG, complement). Useful in evaluation of autoimmune hemolytic anemias, transfusion reactions and erythroblastosis fetalis.

Specimen Collection Requirements

Collect

Two full 4.0 mL lavender (EDTA) top tubes Infants: one lavender (EDTA) top microtainer tube

Handling

Tubes must be labeled appropriately. Refer to Blood Bank Specimen Collection Guidelines. Diagnosis, transfusion history and all medications received by patient should be included on the requisition.

Preferred Volume

Two full 4.0 mL lavender (EDTA) top tubes

Minimum Volume

One lavender (EDTA) top microtainer tube (Whole Blood 0.5 minimum draw)

Transport

Ambient or refrigerated

Rejection Criteria

Gross hemolysis, incorrect specimen container, incorrectly labeled or frozen

Stability

Ambient: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Testing Information

Performed

7 days/week

Reported

30 minutes - 24 hours for routine testing

Method

Antiglobulin testing

Reference Values

Reference Values

Negative

Comments

Drugs including the penicillins and cephalosporins, Aldomet, Levodopa, quinidine, insulin, mefenamic acid, sulfamides, tetracyclines and other drugs may cause a positive test. If indicated, an antibody screen (indirect antiglobulin test) and antibody identification are performed. Elution testing may also be included.

Limitations: Relatively insensitive

CPT Codes

86880

Last Updated: Wednesday, March 15, 2017 04:05:04 PM


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