MyHealth

Manage your account, request prescriptions, set up appointments & more.

LOG IN
Don't have an account
Contact Us

DOUBLE-STRANDED DNA (DSDNA) ANTIBODY, IGG BY ELISA WITH REFLEX TO DSDNA ANTIBODY, IGG BY IFA

Mnemonic

DNA-DS

Synonym

Anti-dsDNA Antibody - DNA - dsDNA Antibody

Guidelines

Secondary screening for SLE based on ANA results.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) top tube

Handling

Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.0 mL serum to an aliquot tube.

Preferred Volume

1.0 mL

Minimum Volume

0.5 mL

Transport

Refrigerated (2-8°C)

Rejection Criteria

Plasma. Contaminated, hemolyzed, or severely lipemic specimens.

Stability

AFTER SEPARATION FROM CELLS: Ambient (20-25°C): 48 hours; Refrigerated (2-8°C): 2 weeks; Frozen (at or below -20°C): 1 year (avoid repeated freeze/thaw cycles)

Testing Information

Performed

Sun-Sat

Reported

2-5 days

Method

Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody

Referral Lab

ARUP (50215)

Reference Values

Reference Values

Interpretation provided.

Comments

Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA.

CPT Codes

86225; if reflexed, add 86256

Last Updated: Monday, November 3, 2014 04:00:41 PM


Client Services: 503-413-1234