DOUBLE-STRANDED DNA (DSDNA) ANTIBODY, IGG BY ELISA WITH REFLEX TO DSDNA ANTIBODY, IGG BY IFA
Anti-dsDNA Antibody - DNA - dsDNA Antibody
Secondary screening for SLE based on ANA results.
Specimen Collection Requirements
Serum, one 5.0 mL gold (SST) top tube
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1.0 mL serum to an aliquot tube.
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.
AFTER SEPARATION FROM CELLS: Ambient (20-25°C): 48 hours; Refrigerated (2-8°C): 2 weeks; Frozen (at or below -20°C): 1 year (avoid repeated freeze/thaw cycles)
Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA.
86225; if reflexed, add 86256
Monday, November 3, 2014 04:00:41 PM
Client Services: 503-413-1234