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ELECTROPHORESIS, SERUM PROTEIN, WITH REFLEX IMMUNOFIXATION

Mnemonic

PELP R

Synonym

Serum Protein Electrophoresis with Reflex Immunoelectrophoresis - SPEP- IEP- IFE- IFX- IFIX

Includes

Total Protein, Protein Electrophoresis

If indicated: Specific protein identification by Immunofixation and Immunoglobulins G, A, M

Specimen Collection Requirements

Collect

Serum, Two 5.0 mL gold (SST) or 7.0 mL red top tubes. Fresh samples are recommended.

View image for Supply Item Number - 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Centrifuge and separate serum from cells within 1 hour of collection.

Preferred Volume

4.5 mL

Minimum Volume

2.0 mL Serum (5.0 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 6 hours; Refrigerated (2-8°C): 7 days; Frozen (≤ -20°C): 1 month

Testing Information

Performed

Sun - Fri

Reported

2-5 days

Method

Helena SPIFE 3000 Protein Electrophoresis, Sebia Hydrasys Immunofixation, Siemens BN ProSpec Nephelometry, Beckman AU Colorimetry

Reference Values

Reference Values

Professional interpretation provided by Cascade Pathology Services.

CPT Codes

84155: Total Protein; 84165: Protein Electrophoresis; 86334: Immunoelectrophoresis, if indicated; 82784(X3): IgG, IgA, and IgM, if indicated

Last Updated: Friday, May 11, 2018 06:30:28 AM


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