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ELECTROPHORESIS, SERUM PROTEIN, WITH REFLEX IMMUNOFIXATION

Mnemonic

PELP R

Synonym

Serum Protein Electrophoresis with Reflex Immunoelectrophoresis - SPEP- IEP- IFE- IFX- IFIX

Includes

Total Protein, Protein Electrophoresis

If indicated: Specific protein identification by Immunofixation and Immunoglobulins G, A, M

Guidelines

Effective August 30, 2016, Legacy Laboratory Services updated reference ranges for serum protein electrophoresis due to an instrumentation change.

For more information, refer to the Legacy LabAlert: New Reference Ranges for Protein Electrophoresis.

Specimen Collection Requirements

Collect

Serum, Two 5.0 mL gold (SST) or 7.0 mL red top tubes. Fresh samples are recommended.

View image for Supply Item Number - 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Centrifuge and separate serum from cells within 1 hour of collection.

Preferred Volume

4.5 mL

Minimum Volume

2.0 mL Serum (5.0 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 6 hours; Refrigerated (2-8°C): 7 days; Frozen (≤ -20°C): 1 month

Testing Information

Performed

Sun - Fri

Reported

2-5 days

Method

Protein Electrophoresis

Immunofixation by Sebia Hydrasys, BN ProSpec Nephelometry

Reference Values

Reference Values

Professional interpretation provided by Cascade Pathology Services.

CPT Codes

84155: Total Protein; 84165: Protein Electrophoresis; 86334: Immunoelectrophoresis, if indicated; 82784(X3): IgG, IgA, and IgM, if indicated

Last Updated: Thursday, January 5, 2017 03:40:59 AM


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