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HEMOGLOBIN ELECTROPHORESIS

Mnemonic

HGB ELP

Synonym

Hemoglobinopathy Evaluation

Includes

Hgb A2, Hgb F included if indicated, HRBC, HRBC morphology, other hemoglobins as detected

Guidelines

Confirmation testing will not be performed on patient samples for which a hemoglobin variant(s) has been previously identified, unless the gel pattern by the primary testing method indicates a change from previous results.

Specimen Collection Requirements

Collect

Whole blood, one 4.0 mL and one 2.0 mL lavender (EDTA) or two 2.0 mL lavender (EDTA) top tubes

Handling

Do not separate. Do not freeze.

Preferred Volume

6.0 mL EDTA Whole Blood

Minimum Volume

1.5 mL EDTA Whole Blood

Transport

Refrigerated (2-8°C)

Rejection Criteria

Clotted or frozen specimens

Stability

UNSPUN SAMPLES:  Room Temperature (18-26°C): Unacceptable; Refrigerated (2-8°C): 7 days; Frozen (≤ -20°C): Unacceptable

Testing Information

Note

For specimens more than 24 hours old, the HRBC and HRBC MORPH will not be performed, and will not be included.

If a lab other than Legacy performs the CBC with the red cell morphology and iron studies, submit a copy of the CBC with the red cell morphology and iron studies.

Performed

As needed, once/week

Reported

2-7 days

Method

Electrophoresis, Column Chromatography, Citrate Agar Electrophoresis as needed, and Automated Cell Counter.
Quantitative HbF, if indicated, is referred to a reference lab and result is used in the calculation of hemoglobin variants.

Reference Values

Reference Values

Professional interpretation provided by Cascade Pathology Services.

Hemoglobin F, Quantitative (if indicated)

CPT Codes

83020

Last Updated: Friday, September 15, 2017 08:53:10 AM


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