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HEPARIN-INDUCED THROMBOCYTOPENIA (HIT) ANTIBODY, PF4 IGG BY ELISA WITH REFLEX TO SEROTONIN RELEASE ASSAY (HEPARIN DEPENDENT PLATELET ANTIBODY), UNFRACTIONATED HEPARIN

Mnemonic

HIT ABR

Synonym

Drug Associated Anti-Platelet Antibody - Heparin Associated Thrombocytopenia - HAT - HIT - Heparin Induced Platelet Antibody - Platelet Factor 4

Guidelines

For a clinical scoring system to assess pretest probability of HIT and other guidance for diagnosing HIT, refer to: Adult Legacy Heparin Induced Thrombocytopenia (HIT) Guidelines

Specimen Collection Requirements

Collect

Serum, one red (non-SST) top tube is preferred. Light blue (3.2% Buffered Sodium Citrate) top tube is acceptable.

Handling

Centrifuge and separate serum or sodium citrated plasma from cells within 1 hour post collection. A minimum of two 1.0 mL aliquots are required.

Preferred Volume

Three 1.0 mL aliquots

Minimum Volume

Two 1.0 mL aliquots

Transport

Frozen, (minus 20°C or below), is preferred method of transport.

NOTE: If client is unable to freeze, serum or sodium citrated plasma aliquots may be transported refrigerated (2-8°C). Aliquot tubes must be frozen immediately when received at Central Laboratory.

Rejection Criteria

Heparinized Microbially contaminated, hemolyzed, lipemic or heat-inactivated serum or sodium citrated plasma.

Serum from serum separator tubes (SST) is not acceptable.

Specimens stored at room temperature are unacceptable.

Stability

AFTER SEPARATION FROM CELLS: Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 1 month

Testing Information

Note

Positive ELISA results are relatively non-specific for a clinical diagnosis of HIT. If ELISA result is positive, then Serotonin Release Assay (a functional assay with greater specificity for a clinical diagnosis of HIT syndrome) will be added. Additional charges apply.

Performed

Testing performed once a day, Monday through Friday. Specimen must be received in the Special Coagulation lab before 9:00 am to be included in daily run.

Reported

HIT ELISA: 1-3 days

SRA: 2-12 days, when reflexed

Method

Enzyme-Linked Immunosorbent Assay/Serotonin Release Assay

Reference Values

Reference Values

0.399 OD or less

CPT Codes

86022; if reflexed add 86022

Last Updated: Thursday, January 5, 2017 03:40:26 AM


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