HEPATITIS B VIRUS DNA ULTRASENSITIVE QUANTITATIVE REAL-TIME PCR

Mnemonic

HBVUPCR

Synonym

Hepatitis B Viral Load

Specimen Collection Requirements

Collect

Serum or Plasma, one gold (SST) or lavender (EDTA) top tube

Handling

Separate serum or plasma from cells within 24 hours. Separate specimens must be submitted when multiple tests are ordered.

Preferred Volume

2.00 mL

Minimum Volume

0.75 mL

Transport

Frozen

Rejection Criteria

Heparinized specimens, nonfrozen specimens or specimens exposed to repeated freeze/thaw cycles

Stability

COLLECTION TO INITIATION OF TESTING ON CELLS: Ambient: 24 hours
AFTER SEPARATION FROM CELLS: Ambient: 72 hours; Refrigerated: 1 week; Frozen: 6 weeks

Testing Information

Note

Specimens received with less than minimum volume for testing will automatically be run with a dilution according to the guidelines below:
-Specimens with 325-650µL will be diluted resulting in a modification of the quantitative range of the assay to 1.6-8.5 log IU/mL (41-350,000,000 IU/mL).
-Specimens with 130-325µL will be diluted resulting in a modification of the quantitative range of the assay to 2.0-8.9 log IU/mL (102-870,000,000 IU/mL).

Performed

7 days/week

Reported

5-7 days

Method

Real-Time PCR

Referral Lab

ARUP (56025)

Reference Values

Reference Values

The quantitative range of this assay is 1.3-8.2 log IU/mL (20-170,000,000 IU/mL).

1 IU/mL of HBV DNA is approximately 5.82 copies/mL.

CPT Codes

87517

Last Updated: Wednesday, May 7, 2014 11:27:44 AM


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