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IMMUNODEFICIENCY PANEL II

Mnemonic

F IMMUNE 2

Synonym

Immunodeficiency Profile II - Flow Immune Deficiency Profile II - T and B-cell Panel - T, B and NK-cell Panel - CD3 and T-Cell Subsets - CD19 and NK Cell Panel - CD4/CD8 - Immunophenotyping - CD3 - CD4 - CD8 - Lymphocyte Quantitation ( T, B and NK)

Includes

CBC, percentage and absolute numbers of total T-cells, helper and suppressor T-cells, B-cells, NK cells and CD3CD4/CD3CD8 ratio

Specimen Collection Requirements

Collect

Whole Blood, one 4.0 mL lavender (Flow) AND one 2.0 mL lavender (Hematology) EDTA top tube; minimum ½ full

Handling

Store at room temperature. Must be received in the lab within 12 hours of collection. CBC must be run within 12 hours and Flow testing must be run within 5 days.

Preferred Volume

Two lavender EDTA tubes; one each, 4.0 mL and 2.0 mL EDTA tube, minimum ½ full

Minimum Volume

1.0 mL whole blood

Transport

Room Temperature

Rejection Criteria

Specimens more than five days old (ambient); refrigerated, clotted, hemolyzed or frozen.

Note: CBC rejection criteria differs, refer to entry for CBC.

Stability

Ambient (20-25°C) – Immune Panel II stable 5 days; CBC stable 12 hours.

Refrigerated and Frozen: Unacceptable.

Testing Information

Performed

Mon - Fri except holidays

Reported

within 24  hours

Method

Flow Cytometry

Reference Values

Reference Values

CD3+%: 59-81 
Absolute CD3:  785-2630/µL 
CD3+CD4+%: 38-65
Absolute CD3+CD4:     510-1835/µL
CD3+CD8+%: 10-37
Absolute CD3+CD8: 120-930/µL
CD19+%: 5-27 
Absolute CD19: 65-760/µL 
CD16+CD56+%: 5-25 
Absolute CD16+CD56: 105-675µL 
CD3CD4/CD3CD8: 1.0-6.5

Comments

Results include Antibody Specificity.

CPT Codes

86355, 85025

Last Updated: Thursday, January 5, 2017 03:40:27 AM


Client Services: 503-413-1234