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IMMUNOELECTROPHORESIS, SERUM

Mnemonic

IEP

Synonym

Serum immunofixation - IFE - IFIX - IEP

Includes

Total Protein, IgA, IgG, IgM, Protein Electrophoresis, Specific Protein Identification by Immunofixation

Specimen Collection Requirements

Collect

Serum, Two 5.0 mL gold (SST) or 7.0 mL red top tubes. Fresh samples are recommended.

Handling

Allow serum to clot completely at room temperature (minimum: SST – 30 minutes and red top tubes – 60 minutes). Centrifuge and separate serum from cells within 1 hour of collection.

Preferred Volume

4.5 mL

Minimum Volume

2.0 mL Serum (5.0 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 6 hours; Refrigerated (2-8°C): 7 days; Frozen
 (≤ -20°C): 1 month 
 

Testing Information

Performed

Sun - Fri

Reported

2-5 days

Method

Helena SPIFE 3000 Protein Electrophoresis, Sebia Hydrasys Immunofixation, Siemens BN ProSpec Nephelometry, Beckman AU Colorimetry

Reference Values

Reference Values

Professional interpretation provided by Cascade Pathology Services.

CPT Codes

86334, 82784 (X3), 84165, 84155

Last Updated: Friday, May 11, 2018 12:12:52 PM


Client Services: 503-413-1234