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JAK2 EXON 12 MUTATION ANALYSIS BY PCR

Mnemonic

JAK2 EX12

Guidelines

Most appropriate in cases of high suspicion of polycythemia vera with negative JAK2 V617F mutation status.

Specimen Collection Requirements

Collect

Whole Blood, one 5.0 mL lavender (EDTA) top tube or Bone Marrow, one 3.0 mL lavender (EDTA) top tube

Handling

Do not freeze

Preferred Volume

Whole Blood, 5.0 mL or Bone Marrow, 3.0 mL

Minimum Volume

1.0 mL

Transport

Refrigerated

Rejection Criteria

Frozen or clotted samples, serum or severely hemolyzed whole blood, specimens collected in anticoagulants other than EDTA

Stability

Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable

Testing Information

Performed

DNA Isolation: Sun - Sat

Assay: Mon, Wed, Fri

Reported

8-12 days

Method

Polymerase Chain Reaction

Referral Lab

ARUP (2002357)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

81403

Last Updated: Thursday, September 28, 2017 07:45:41 AM


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