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* KLEIHAUER-BETKE

Mnemonic

KLEIHAUER

Synonym

Hemoglobin F Stain - Kleihauer

Specimen Collection Requirements

Collect

Whole Blood: one 4.0 mL or 2.0 mL lavender (EDTA) top tube, minimum 1/2 full; or one micro lavender (EDTA) top container, minimum 1/2 full. Amniotic Fluid: one 4.0 mL or 2.0 mL lavender (EDTA) top tube, minimum 1/2 full. Must specify specimen type when amniotic fluid is the specimen.

Handling

Micro containers are not acceptable for amniotic fluid. Non-anticoagulated specimens are acceptable provided specimen is not clotted. Must meet minimum specimen volumes. The collection container must be filled to a minimum of one half of the maximum sample volume (i.e. 4.0 mL tube must have 2.0 mL of blood). The collection tubes must not be filled to more than the maximum sample volume. Blood should be processed within 24 hours of collection. EDTA is the preferred specimen with a 2 week stability. Oxalate is acceptable, but is only stable for 24 hours. Refrigerate at 2-8°C.

Preferred Volume

1.0 mL

Minimum Volume

0.5 mL

Transport

Whole Blood: Refrigerate at 2-8°C; Amniotic Fluid: Refrigerate at 2-8°C

Rejection Criteria

Under filled tube, overfilled tube, frozen, clotted or grossly hemolyzed samples, incorrect anticoagulant

Stability

Ambient (18-21°C): 12 hours; Refrigerated (2-8°C): 2 weeks

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Acid Elution/Microscopy

Reference Values

Reference Values

Interpretation provided.

Comments

Do not substitute Fetal Bleed Screen for this test.

CPT Codes

85460

Last Updated: Thursday, January 5, 2017 03:41:20 AM


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