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LACTOSE TOLERANCE

For patient preparation instructions, refer to Lactose Tolerance Tests-Patient Preparation Instructions

Mnemonic

LACT TOL

Synonym

Lactose Tolerance Test used to Diagnose Lactose Malabsorption - Disaccharide Found in Milk - Lactase Deficiency

Includes

Fasting, 15, 30, 60, 90 and 120 minute glucose post LAC-TOL

Specimen Collection Requirements

Patient Preparation

Patient should fast for 8-14 hours before administering test. Draw fasting specimen. For adults, administer 50g LAC-TOL (lactose sugar). Pediatric doses vary by child’s weight. Draw specimens at 15, 30, 60, 90 and 120 minutes post sugar.

Collect

Preferred: Plasma, one 4.0 mL gray (sodium fluoride/potassium oxalate) top tube

Also acceptable: Serum or Plasma, one 5.0 mL gold (SST), 7.0 mL red or 3.0 mL mint green (lithium heparin, PST) top tube

Handling

The gold, red and green top tubes must be delivered to the lab immediately. Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Preferably, separate serum or plasma from cells within 1 hour of collection. If unable to spin, preferred specimen is gray (sodium fluoride/potassium oxalate) top tube.

Preferred Volume

1.0 mL Serum/Plasma per specimen

Minimum Volume

0.25 mL Serum/Plasma (Whole Blood 0.60 mL minimum draw) per specimen

Transport

Refrigerated (2-8°C)

Rejection Criteria

Grossly hemolyzed specimens, timed specimens collected outside the ±10 minute window (±5 minutes for 15 minute specimen)

Stability

AFTER SEPARATION FROM CELLS:

SODIUM FLUORIDE/POTASSIUM OXALATE PLASMA: Room Temperature (18-26°C): 24 hours; Refrigerated (2-8°C): 7 days; Frozen (≤ -20°C): 1 month

SERUM OR LITHIUM HEPARIN PLASMA: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 3 days; Frozen (≤ -20°C): 1 month

Testing Information

Performed

Schedule testing with one of the approved Legacy Facilities. Please refer to Lactose Tolerance Tests-Patient Preparation Instructions.

Reported

24 hours

Method

UV spectrophotometry

Reference Values

Reference Values

Interpretation provided.

CPT Codes

For 3 specimens: 82951; For each additional specimen: 82952

Last Updated: Tuesday, January 24, 2017 01:21:26 PM


Client Services: 503-413-1234