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LEUKOCYTE ALKALINE PHOSPHATASE

Mnemonic

LAP

Synonym

LAP - LAP Stain

Specimen Collection Requirements

Collect

BOTH Whole Blood, one dark green (sodium heparin) top tube AND five unfixed, well-made Peripheral Blood Smears.
Peripheral blood smears must be made from sodium heparin tube.

Handling

Whole blood must be received within 8 hours of collection. When unfixed smears are submitted, the slides must be received within 24 hours of preparation. The Lab must be able to stain the slides within 72 hours. The Flow Lab is open Monday through Friday 0800-1800. This test cannot be processed on the weekend. Notify the Flow Cytometry Lab at (503) 413-7358 that a specimen is coming.

Preferred Volume

4.0 mL

Minimum Volume

0.5 mL

Transport

Room temperature (20-25°C)

Rejection Criteria

EDTA specimens, whole blood more than 12 hours old, blood smears more than 24 hours old, frozen specimens, peripheral blood smears from EDTA specimens, peripheral blood smears from specimens older than 8 hours

Stability

WHOLE BLOOD: Ambient: 12 hours; Refrigerated: Unacceptable; Frozen: Unacceptable BLOOD SMEARS: Ambient: 72 hours

Testing Information

Performed

Mon - Fri (0800-1800)

Reported

24 hours, excluding weekends

Method

Manual Cytochemistry

Reference Values

Reference Values

Score 20-137

CPT Codes

85540

Last Updated: Thursday, January 5, 2017 03:41:22 AM


Client Services: 503-413-1234