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LUPUS ANTICOAGULANT PANEL WITH SEROLOGY

Mnemonic

LA PAN SERO

Synonym

Antiphospholipid Panel - Antiphospholipid Antibody (AB) - Lupus Inhibitor - Lupus Anticoagulant

Includes

Guidelines

Relevant patient medical history needs to be submitted with orders for Lupus Anticoagulant testing.  

Electronic orders: Upon ordering, prompts will appear asking about relevant medications such as anticoagulants, antifibrinolytics, recent transfusion history, and history of bleeding or clotting disorders. 

Paper requisition: If a paper requisition is to be used, please include Patient History for Lupus Testing Form

(Click here to view/print form.)

NOTE: Anticoagulant therapy, may cause falsely prolonged PT, PTT and LA Test (dRVVT) test results.

PT or APTT mixing studies are automatically reflexed if the PT and/or APTT are unexplainably abnormal (i.e., the patient is not receiving anticoagulant therapy, does not have a vitamin K deficiency, or have a clinical condition that would cause a prolonged clotting time). LA Check (dRVVT) or 1:1 Mix (dRVVT) are reflexed as indicated.  DRVVT denotes Dilute Russells Viper Venom Test.

Other non-clot based tests may be helpful (i.e. Anticardiolipin, Anti-Beta2-Glycoprotein 1) and are included in this panel.

Specimen Collection Requirements

Collect

Whole Blood light blue (3.2% Buffered Sodium Citrate) AND Serum, gold (SST) top tubes

Handling

Refer to Coagulation Specimen Collection Guidelines. Serum must be separated from cells ASAP. Due to short stability, whole blood specimen tubes must reach the testing department within 4 hours, otherwise submit frozen plasma and serum aliquots. NOTE: Frost-free refrigerator freezers are not acceptable for frozen sample storage. If proper storage is unavailable at the collection site, freeze/store aliquots on dry ice. Alternatively, send the patient to the nearest Legacy Hospital Outpatient Lab for sample collection/processing.

If submitting whole blood, transport at ambient temperature (15-25°C) in original unopened tubes. If submitting frozen plasma and serum aliquots, specimens must remain frozen (-20°C) during transport.

Preferred Volume

Four 2.7 mL whole blood (light blue top) or six 1.0 mL frozen plasma aliquots AND One gold (SST) tube or two 2.0 mL frozen serum aliquots

Minimum Volume

Three 2.7 mL whole blood (light blue tops) or four 1.0 mL frozen plasma aliquot AND One gold (SST) tube or two 2.0 mL frozen serum aliquots

Transport

Whole Blood: Ambient (15-25°C) 

Plasma and Serum: Frozen (-20°C), aliquots must remain frozen during storage and transport

Rejection Criteria

Refrigerated (2-8°C) whole blood sodium citrate tubes or refrigerated (2-8°C) plasma sodium citrate. 

PLASMA: Clotted samples, samples collected in containers other than 3.2% buffered sodium citrate, tubes filled less than 90%, samples from patients with a Hematocrit greater than 55%, hemolyzed samples due to ex-vivo causes (i.e., difficult draw, poor sample draw), whole blood samples that were frozen before processing SERUM: Plasma or other body fluids, severely lipemic, contaminated or severely hemolyzed

Stability

WHOLE BLOOD: Ambient: 4 hours
PLASMA: Ambient: 4 hours; Frozen: (-20°C) 2 weeks, (-70°C) 6 months
SERUM AFTER SEPARATION FROM CELLS: Ambient: 8 hours; Refrigerated: 48 hours; Frozen: 1 year

Testing Information

Performed

LA Test (dRVVT): Daily, Mon-Friday
Cardiolipin Antibody: Tuesday, Thursday
Beta2 Glycoprotein1: Monday, Thursday

Reported

1 week

Method

Clotting (dRVVT)/ EIA/ ELISA

Reference Values

Reference Values

Interpretation provided.

CPT Codes

85610, 85730, 85613, 86146 (x3), 86147 (x3)

Last Updated: Wednesday, May 17, 2017 09:00:26 AM


Client Services: 503-413-1234