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LYMPHOMA/CHRONIC LYMPHOID LEUKEMIA PANEL, BY FLOW CYTOMETRY

Synonym

ATTN FLOW - CLL Panel

Guidelines

This panel is designed to aid in the diagnosis and subcategorization of suspected or known lymphomas or chronic lymphoid leukemia.

Specimen Collection Requirements

Collect

Bone Marrow Aspirate, collect bone marrow in a heparinized syringe and transfer into EDTA tube or into RPMI for transport to the lab. Also include 2 unstained aspirate smears. If absolute lymphocytosis is present, Peripheral Blood, in lavender (EDTA) top tube is preferred, but heparin may be used. Fresh Tissue in RPMI may also be submitted. Ensure that the tissue is completely immersed in the RPMI nutrient media.

Handling

Maintain specimens at room temperature, avoid temperature extremes and do not centrifuge. Call Client Services ASAP at (503) 413-1234 or Toll Free (877) 270-5566 to arrange for PRIORITY pickup.

Always submit recent CBC (collected within past 72 hours), differential and 2 unstained blood smears with request, for both bone marrow or blood specimens.

For complete collection and handling guidelines see Cytogenetics/Flow Cytometry/FISH Specimens for Leukemia, Lymphoma and Other Oncology Referrals

Preferred Volume

Bone Marrow: 4.0 mL, Blood: 4.0 mL

Minimum Volume

Bone Marrow: 0.5 mL, Blood: 2.0 mL. Must have sufficient number of malignant cells for analysis.

Transport

Room temperature (20-25°C)

Rejection Criteria

Bone Marrow: Never rejected without consultation with Hematopathologist. Blood: Clotted, severely hemolyzed or frozen specimens

Stability

Ambient: 24 hours; Refrigerated: 24 hours; Frozen: Unacceptable

Testing Information

Note

Bone Marrow Requisitions and supplies including RPMI are available upon request.

Performed

Mon - Fri (0800-1900), by special arrangements on Sat

Reported

24 hours

Method

Flow Cytometry

Reference Values

Reference Values

Interpretation provided.

CPT Codes

88184, 88185, 88189

Last Updated: Tuesday, June 6, 2017 11:12:41 AM


Client Services: 503-413-1234