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METHADONE, QUANTITATIVE, SERUM

Mnemonic

METHAD

Synonym

Dolophine

Specimen Collection Requirements

Collect

Serum or Plasma, one 7.0 mL red, 4.0 mL dark green (sodium heparin) or 4.0 mL Lavender (EDTA) top tube

Handling

Allow specimen to clot completely at room temperature (minimum: 30 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

2 mL Serum or Plasma

Minimum Volume

1 mL

Transport

Refrigerated (2-8°C)

Rejection Criteria

Serum that has been in contact with serum separator gel

Stability

Ambient (20-25°C): 1 week; Refrigerated (2-8°C): 1 month; Frozen (at or below -20°C): 3 months

Testing Information

Performed

Tue - Sat

Reported

24-48 hours

Method

Liquid Chromatography Tandem Mass Spectrometry

Reference Values

Reference Values

Methadone: Not Detected  < 30 ng/mL

Therapeutic Range

Toxic Levels

Comments

Interpretation: Reported therapeutic and toxic serum Methadone levels have significant overlap.

CPT Codes

80358

Last Updated: Friday, March 10, 2017 11:46:04 AM


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