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PML-RARA TRANSLOCATION, t(15;17) BY RT-PCR, QUANTITATION

Mnemonic

PML QNT

Synonym

t(15;17)

Guidelines

The t(15;17) chromosomal translocation which leads to the PML/RARα fusion transcript is strongly associated with acute promyelocytic leukemia (APL).  The detection of this highly characteristic abnormality is useful in the accurate diagnosis of APL.  These tumor-specific markers can also be used to monitor the remission status of affected patients.  RT-PCR provides a rapid alternative to cytogenetic analysis and may identify PML/RARα gene rearrangement in the absence of detectable t(15;17) by the latter technique.

Specimen Collection Requirements

Collect

Whole Blood or Bone Marrow, one 5.0 mL lavender (EDTA) or 3.0 mL Bone Marrow in EDTA

Handling

Specimens must be received at ARUP within 48 hours of collection. Draw specimens Monday - Thursday only.

Preferred Volume

5.0 mL whole blood or 3.0 mL bone marrow in EDTA

Minimum Volume

1.0 mL whole blood or bone marrow in EDTA

Transport

Refrigerated (2-8°C)

Rejection Criteria

Serum or plasma, frozen or clotted whole blood or bone marrow, specimens collected in preservatives other than EDTA, severely hemolyzed specimens

Stability

Ambient: 1 hour; Refrigerated: 2 days; Frozen: Unacceptable

Testing Information

Performed

RNA Isolation: 7 days/week Assay: Varies

Reported

3-8 days

Method

Polymerase Chain Reaction

Referral Lab

ARUP (2002871)

Reference Values

Reference Values

Negative

CPT Codes

81315

Last Updated: Thursday, January 5, 2017 03:41:47 AM


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