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QUANTIFERON GOLD TB

Mnemonic

QF GOLD TB

Synonym

QFT - QuantiFERON® Gold-TB In Tube - QFT ELISA - QF GOLD - QFT-IT

Specimen Collection Requirements

Collect

PLASMA; collected using the QuantiFERON® TB Collection Kit

(View specimen collection kit)

Handling

The 3 collection tubes must be at 17-25°C at the time of the blood filling. Collect 1 mL of blood by venipuncture directly into each QFT tube. If using a butterfly collection device, prime tubing with a purge tube before filling the QFT tubes. The QFT collection tubes fill slowly so hold the tube on the needle for 2-3 seconds after flow ceases. Fill each tube to the black mark, 0.8 mL to 1.2 mL. Immediately after filling the tubes, shake them 10 times, firmly enough to ensure the entire inner surface of the tubes are coated with blood.

Label each tube, placing the patient label on the tube so as to not block visibility of the black line on the tube. Keep tubes at room temperature (22°C ±5°C) and transport to the laboratory within 16 hours of collection.

Minimum Volume

Each of the 3 tubes included in the Collection kit must fill to the 1 mL mark

Transport

The 3 tubes must be labeled and transported at room temperature (22°C ±5°C) and MUST arrive at the Central Laboratory within 16 hours of collection.

Rejection Criteria

Refrigerated or frozen specimens, specimen tubes with incorrect volume and specimens that arrive in the laboratory more than 16 hours post collection

Stability

Ambient: 16 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Testing Information

Note

Upon arrival in the Laboratory, specimens must be incubated for 16-24 hours prior to test performance.

Performed

Tue, Thur, Sun

Reported

7 days

Method

ELISA

Reference Values

Reference Values

Negative. For interpretation see the reference values.

Comments

QFT is a qualitative diagnostic test measuring in vitro interferon gamma response to peptide antigens simulating mycobacterial proteins.  The magnitude of response cannot be correlated to stage or degree of infection, level of immune responsiveness, or likelihood for progression to active disease.  Diagnosing or excluding tuberculosis, requires a combination of epidemiological, historical, medical and diagnostic findings that should be taken into account when interpreting QFT results.

For detailed recommendations, refer to CDC Guidelines for using InterFeron Gamma Release Assays to detect M. tuberculosis infection, Jan, 2010

CPT Codes

86480

Last Updated: Thursday, January 5, 2017 03:41:34 AM


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