The 3 collection tubes must be at 17-25°C at the time of the blood filling. Collect 1 mL of blood by venipuncture directly into each QFT tube. If using a butterfly collection device, prime tubing with a purge tube before filling the QFT tubes. The QFT collection tubes fill slowly so hold the tube on the needle for 2-3 seconds after flow ceases. Fill each tube to the black mark, 0.8 mL to 1.2 mL. Immediately after filling the tubes, shake them 10 times, firmly enough to ensure the entire inner surface of the tubes are coated with blood.
Label each tube, placing the patient label on the tube so as to not block visibility of the black line on the tube. Keep tubes at room temperature (22°C ±5°C) and transport to the laboratory within 16 hours of collection.