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RhCc ANTIGEN (RHCE) GENOTYPING

Synonym

Rh Genotyping C/c Antigen

Specimen Collection Requirements

Collect

Cultured Cells (preferred): Two T-25 flasks at 80% confluent of cultured amniocytes, Amniotic Fluid: 5-10 mL unspun

Handling

CRITICAL AMBIENT. Fill flasks with culture media to ship. Backup cultures must be retained at the clients institution until testing is completed. Samples must be received within 24 hours of collection at Legacy Central Lab by noon Monday through Friday.

Preferred Volume

Two T-25 flasks at 80% confluent cultured amniocytes, 10.0 mL unspun amniotic fluid, 3.0 mL whole blood

Minimum Volume

Amniotic Fluid:  5 mL
Cultured Amniocytes:
Two T-25 flasks
Maternal Cell Contamination Specimen:
1 mL
Whole Blood (Parental Genotyping):
1 mL

Transport

Amniotic fluid: Room temperature.
Cultured Amniocytes:
 CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells.
Whole Blood or Maternal Cell Contamination Specimen:
 Refrigerated.

Stability

Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Whole Blood or Maternal Cell Contamination Specimen:
Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable

Testing Information

Performed

Tue, Thur, Sat

Reported

2-7 days

Method

Polymerase Chain Reaction/Fluorescent Monitoring

Referral Lab

ARUP (50421)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

81403

Last Updated: Thursday, January 5, 2017 03:41:35 AM


Client Services: 503-413-1234