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*SICKLE CELL SCREEN

Mnemonic

SICKLE TST

Synonym

Hemoglobin S Solubility - HbS

Guidelines

This is not a diagnostic test for sickle cell trait or disease. Positive results require further studies and interpretation.

Blood samples from infants and from newborns, up to six months of age, can have up to 25% HgbF present which can cause false negatives. Sickle cell solubility testing is not performed on patients less than 6 months of age. Hemoglobin electrophoresis may be ordered as an alternate testing method.

Specimen Collection Requirements

Collect

Whole Blood: one 4.0 mL or 2.0 mL lavender (EDTA) tube, minimum 1/2 full

Pediatric: one micro lavender (EDTA) top tube, minimum 1/2 full

Handling

EDTA is the preferred anticoagulant but the following are also acceptable: heparin, ACD, CPD, Oxalate. The collection container must be filled to a minimum of 1/2 the maximum sample volume (i.e. 4.0 mL tube must have 2.0 mL blood). The collection tube must not be filled to more than the maximum sample volume.

Preferred Volume

Whole Blood, 2.0 - 4.0 mL

Minimum Volume

Whole Blood, the collection container must be filled to a minimum of 1/2 of the maximum sample volume, i.e.

  • 4.0 mL tube must have 2.0 mL of blood minimum
  • 2.0 mL tube must have 1.0 mL of blood minimum
  • Micro lavender (EDTA) top container minimum ½ full

Transport

Refrigerate at 2-8°C

Rejection Criteria

Specimens collected on patients less than 6 months of age. Under filled tube, over filled tube, moderate to severe hemolysis, clotted or frozen specimens.

Stability

Ambient: (20-25°C) 12 hours; Refrigerated (2-8°C): Up to 6 weeks

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Commercial Solubility Kit

Reference Values

Reference Values

Negative

CPT Codes

85660

Last Updated: Thursday, January 5, 2017 03:41:37 AM


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