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SYPHILIS CONFIRMATION WITH REFLEX, REVERSE ALGORITHM

Mnemonic

SYPH CONF RFLX

Synonym

RPR - TP-PA - Treponemal Test - Non-treponemal Test - Syphilis Reverse Algorithm

Includes

First confirmation:

  • RPR Confirmation with Reflex to Titer & TP-PA

Possible second confirmation, if indicated:

  • T. pallidum Antibody by TP-PA

Guidelines

Please see Syphilis Testing Algorithms

Order test if initial immunoassay screen was performed by a separate laboratory.

Syphilis Confirmation with Reflex assumes that an immunoassay screen has been performed at a separate laboratory.  This test is used to confirm initial reactive/positive testing used in the reverse syphilis screen. The interpretive statement is valid only for specimens having previously been screened and found to be reactive by a Treponemal antibody immunoassay screen.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

View image for Supply Item Number 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Separate serum from cells within 1 hour of collection.

Preferred Volume

1.5 mL Serum

Minimum Volume

0.75 mL Serum (1.8 mL minimum whole blood draw)

Transport

Refrigerated (2-8 ºC)

Rejection Criteria

Hemolyzed specimens, Contaminated with bacteria, Grossly lipemic or turbid specimens, Plasma, CSF

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 24 hours; Refrigerated (2-8°C): 5 days; Frozen (≤ -20°C): 2 months

Testing Information

Performed

RPR: 7 days/week

TP-PA: Mon-Fri

Reported

1-4 days

Method

First confirmation:

  • RPR Cardiolipin Card Flocculation Assay

Second confirmation, if indicated:

  • T. Pallidum Particle Agglutination

Reference Values

Reference Values

Interpretation provided.

Comments

Reactive RPR results will be titered. Non-reactive RPR results will be reflexed to T. Pallidum Antibody by TP-PA for confirmation.

CPT Codes

RPR: 86592; RPR Titer: 86593; TP-PA: 86780

Last Updated: Thursday, July 27, 2017 10:17:12 AM


Client Services: 503-413-1234