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SYPHILIS ANTIBODY SCREEN WITH REFLEX

Mnemonic

SYPH AB RFLX

Synonym

Syphilis Screen with Reflex, Reverse Algorithm - RPR - TP-PA - Treponemal Test - Non-treponemal Test

Includes

Screen: Syphilis Antibody by CIA

Possible confirmation, if indicated:

  • RPR Confirmation with Reflex to Titer and TP-PA
  • T. pallidum Antibody by TP-PA

Guidelines

Please see Syphilis Testing Algorithms

Order test for patients greater than or equal to 30 days old, including pregnant females.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

View image for Supply Item Number 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Separate serum from cells within 1 hour of collection.

Preferred Volume

3.0 mL Serum

Minimum Volume

1.5 mL Serum (3.5 mL minimum whole blood draw)

Transport

Refrigerated (2-8 ºC)

Rejection Criteria

Hemolyzed specimens, Contaminated with bacteria, Grossly lipemic or turbid specimens, Plasma, CSF

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 24 hours; Refrigerated (2-8°C): 5 days; Frozen (≤ -20°C): 1 month

Testing Information

Performed

Syphilis Screen: 7 days/week

RPR: 7 days/week

TP-PA: Mon-Fri

Reported

1-5 days

Method

Screen: Chemiluminescent Immunoassay

Possible confirmation, if indicated:

  • RPR Cardiolipin Card Flocculation Assay
  • T. Pallidum Particle Agglutination

Reference Values

Reference Values

Interpretation provided.

Comments

Reactive Syphilis Antibody by CIA results are automatically reflexed to RPR. Reactive RPR results will be titered. Non-reactive RPR results will be reflexed to T. Pallidum Antibody by TP-PA for confirmation.

CPT Codes

Syphilis Ab: 86780; RPR: 86592; RPR Titer: 86593; TP-PA: 86780

Last Updated: Thursday, July 27, 2017 10:15:32 AM


Client Services: 503-413-1234