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TESTOSTERONE, FREE, FEMALES OR CHILDREN

Mnemonic

TESTOS FFC

Includes

Free Testosterone

Guidelines

Perform on females of all ages and males < 16 years old. For adult males, order Testosterone, Free, Adult Males.

Specimen Collection Requirements

Patient Preparation

Morning specimen collected between 6-10 AM is preferred.

Collect

Serum or Plasma, one 5.0 mL gold (SST), 3.0 mL mint green (lithium heparin, PST), or dark green (sodium heparin) top tube

View Image for Supply Item Number - 101235

Handling

Allow serum to clot completely at room temperature (minimum - SST: 30 minutes). Centrifuge and separate serum and plasma from cells ASAP or within 2 hours of collection. Transfer 1.0 mL serum or plasma to an aliquot tube.

Preferred Volume

1.0 mL Serum/Plasma

Minimum Volume

0.8 mL Serum/Plasma (1.8 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

EDTA plasma

Stability

AFTER SEPARATION FROM CELLS: Ambient (18-26°C): 24 hours; Refrigerated (2-8°C): 1 week; Frozen (< -20°C): 6 months

Testing Information

Performed

7 days/week

Reported

2-5 days

Method

Free Testosterone: Calculation from Total Testosterone and SHBG
Total Testosterone: LC/MS/MS
SHBG: Chemiluminescent Immunoassay

Referral Lab

ARUP (81059)

Reference Values

Reference Values

Interpretation provided.
Age related reference ranges.

Comments

The concentration of free testosterone is derived from a mathematical expression based on the constant for the binding of testosterone to SHBG.

CPT Codes

84402

Last Updated: Thursday, January 5, 2017 03:40:26 AM


Client Services: 503-413-1234