MyHealth

Manage your account, request prescriptions, set up appointments & more.

LOG IN
Don't have an account
Contact Us

VITAMIN D, 25-HYDROXY

Specimens from patients less than 1 year old will be sent to Mayo Medical Laboratories. A form of 25-Hydroxyvitamin D found significantly in infants can interfere with accurate measurement and interpretation of Vitamin D status.

Mnemonic

VIT D25L (if ≥1 year); VIT D25 1YR (<1 year)

Synonym

Vitamin D - 25-Hydroxyvitamin D2 - 25-Hydroxyvitamin D3 - Calciferol, 25-Hydroxycalciferol - Cholecalciferol - Ergocalciferol

Includes

Total 25-Hydroxyvitamin, 25-Hydroxyvitamin D2, and 25-Hydroxyvitamin D3

Guidelines

Tandem mass spectrometry measures 25(OH)D2 and 25(OH)D3 directly. The total is calculated by adding the two together.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

Supply Item Number - 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: 30 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

1.0 mL

Minimum Volume

0.5 mL Serum, (Whole Blood 1.3 mL minimum draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Gross hemolysis

Stability

AFTER SEPARATION FROM CELLS: Ambient (18-26°C): 12 hours; Refrigerated (2-8°C): 3 days; Frozen (at or below -20°C): 6 months

Testing Information

Performed

Mon - Sat

Reported

1-2 days

Method

LC/MS/MS

Reference Values

Reference Values

Severely deficient:    0-14 ng/mL
Mildly deficient:       15-31 ng/mL
Optimal:                   32-100 ng/mL
Consider toxicity:    >100  ng/mL

CPT Codes

82306

Last Updated: Thursday, January 5, 2017 03:41:43 AM


Client Services: 503-413-1234