SYPHILIS SCREEN WITH REFLEX REVERSE ALGORITHM

Mnemonic

SYPH AB RFLX

Synonym

Syphilis Screen with Reflex, Reverse Algorithm - RPR - TP-PA - Treponemal Test - Non-treponemal Test

Includes

Screen: Syphilis Antibody by CIA

Possible confirmation, if indicated:

  • RPR Confirmation with Reflex to Titer and TP-PA
  • T. pallidum Antibody by TP-PA

Guidelines

Please see Syphilis Testing Algorithms

Test is for patients ≥30 days old, including pregnant females

For patients <30 days old, see Syphilis Screen Neonatal

For CSF testing, see VDRL, Cerebrospinal Fluid

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

View image for Supply Item Number 101235 or 101192

Handling

Allow serum tubes to clot completely at room temperature (minimum - SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells within 24 hours of collection.

Preferred Volume

3.0 mL Serum

Minimum Volume

1.5 mL Serum (4.5 mL minimum whole blood)

Transport

Refrigerated (2-8 ºC)

Rejection Criteria

  • Grossly hemolyzed or plasma specimens
  • Grossly lipemic or turbid specimens
  • CSF

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 6 days; Refrigerated (2-8°C): 6 days; Frozen (≤ -20°C): 1 month

Testing Information

Performed

7 days/week

Reported

24-48 hours

Method

Screen: Chemiluminescent Immunoassay

Possible confirmation, if indicated:

  • RPR Cardiolipin Card Flocculation Assay
  • T. Pallidum Particle Agglutination

Reference Values

Reference Values

Non-Reactive

Please see Syphilis Testing Algorithms

Comments

Reactive Syphilis Antibody by CIA results are automatically reflexed to RPR. Reactive RPR results will be titered. Non-reactive RPR results will be reflexed to T. Pallidum Antibody by TP-PA for confirmation.

CPT Codes

Syphilis Ab: 86780; RPR: 86592; RPR Titer: 86593; TP-PA: 86780

Last Updated: Thursday, January 5, 2017 03:41:59 AM