ALPHAFETOPROTEIN, SERUM Mnemonic AFP Synonym AFP Serum - Tumor Marker - alpha-fetoprotein Guidelines For Prenatal Screening, Second Trimester, see MS AFP and MS QUAD Specimen Collection Requirements Collect Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube Supply Item Number - 101235 or 101192 Handling Allow serum tubes to clot completely at room temperature (minimum - SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells ASAP. Preferred Volume 1.0 mL Serum Minimum Volume 0.5 mL Serum (1.2 mL minimum whole blood draw) Transport Refrigerated (2-8°C) Rejection Criteria Grossly hemolyzed or plasma specimens Stability AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 14 days; Frozen (≤ -20°C): 2 months Testing Information Performed Mon - Fri and a single run on the weekend Reported 24-48 hours Method Chemiluminescent Immunoassay Reference Values Reference Values ADULT MALES and Non-pregnant FEMALES: 0.0-9.0 ng/mL PEDIATRIC RANGES 0 - 1 week: 5,000-105,000 ng/mL 2 weeks - 1 month: 300-60,000 ng/mL 1 month: 100-10,000 ng/mL 2 months: 40-1,000 ng/mL 3 months: 11-300 ng/mL 4 months: 5-200 ng/mL 5 months: 0-90 ng/mL 6 months - Adult: 0.0-9.0 ng/mL CPT Codes 82105 Last Updated: Thursday, January 5, 2017 03:41:56 AM