ALPHAFETOPROTEIN, SERUM

Mnemonic

AFP

Synonym

AFP Serum - Tumor Marker - alpha-fetoprotein

Guidelines

For Prenatal Screening, Second Trimester, see MS AFP and MS QUAD

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

Supply Item Number - 101235 or 101192

Handling

Allow serum tubes to clot completely at room temperature (minimum - SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

1.0 mL Serum

Minimum Volume

0.5 mL Serum (1.2 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Grossly hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 14 days; Frozen (≤ -20°C): 2 months

Testing Information

Performed

Mon - Fri and a single run on the weekend

Reported

24-48 hours

Method

Chemiluminescent Immunoassay

Reference Values

Reference Values

ADULT MALES and Non-pregnant FEMALES:     0.0-9.0 ng/mL
PEDIATRIC RANGES
0 - 1 week:                 5,000-105,000 ng/mL
2 weeks - 1 month:     300-60,000 ng/mL
1 month:                     100-10,000 ng/mL
2 months:                     40-1,000 ng/mL
3 months:                     11-300 ng/mL
4 months:                      5-200 ng/mL
5 months:                      0-90 ng/mL
6 months - Adult:         0.0-9.0 ng/mL

CPT Codes

82105

Last Updated: Thursday, January 5, 2017 03:41:56 AM