ANTI-NUCLEAR ANTIBODY

Mnemonic

ANA

Synonym

ANA

Guidelines

Patients with suspected connective tissue disease might have Antinuclear Antibodies. Disease association may include Systemic Lupus Erythematosus, Sjogren’s Syndrome, Mixed Connective Tissue Disease and Scleroderma.

Specimen Collection Requirements

Collect

Serum, one gold (SST) top tube

Handling

Allow blood tube to sit for 30 minutes to clot. Centrifuge and separate serum from cells immediately. Pour serum into a plastic tube. If multiple tests are requested, please submit in separate transport tube.

Preferred Volume

0.50 mL

Minimum Volume

0.1 mL

Transport

Refrigerated

Rejection Criteria

Plasma, serum with a high degree of hemolysis, lipemia or microbial growth

Stability

Refrigerated: 48 hours; Frozen -20°C for longer storage

Testing Information

Note

If multiple tests are requested, please submit in separate transport tube.

Method

IFA Human Epithelioid Cells (HEp-2)

Referral Lab

Samaritan Health Services Laboratories

Reference Values

Reference Values

Negative: <1:40

CPT Codes

86038 (ANA), 86039 (Titer)

Last Updated: Thursday, January 5, 2017 03:42:00 AM