CATECHOLAMINES, URINE FRACTIONATED FREE

Mnemonic

CATU

Synonym

Adrenaline - Dopamine - Epinephrine - Noradrenaline - Norepinephrine

Specimen Collection Requirements

Patient Preparation

Drugs and medications may affect results and should be discontinued for at least 72 hours prior to specimen collection, if possible. Medications which may physiologically interfere with catecholamines and metabolites include amphetamines and amphetamine-like compounds, appetite suppressants, bromocriptine, buspirone, caffeine, carbidopa-levodopa (Sinemet®), clonidine, dexamethasone, diuretics (in doses sufficient to deplete sodium), ethanol, isoproterenol, methyldopa (Aldomet®), MAO inhibitors, nicotine, nose drops, propafenone (Rythmol), reserpine, theophylline, tricyclic antidepressants, and vasodilators. The effects of some drugs on catecholamine results may not be predictable.

Collect

Urine, 24-hour or random urine collection

Handling

5.0 mL aliquot from a well-mixed 24-hour or random collection. Record the total volume and collection time interval on the transport tube and test request form. Adequate refrigeration is the most important aspect of specimen preservation. Preservation can be enhanced by adjusting the pH to 2-3 by adding an acid such as 6 mol/L hydrochloric acid. Catecholamines are not stable above pH 7. The pH of such specimens must be adjusted by the addition of acid before transport. A pH less than 2 can cause assay interference.

Preferred Volume

4.0 mL

Minimum Volume

2.5 mL

Transport

Refrigerated

Rejection Criteria

Specimens preserved with boric acid or acetic acid. Specimens with pH greater than 7.

Stability

Unpreserved: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Undefined

Preserved: Ambient: Unacceptable; Refrigerated: 1 month; Frozen: 6 months

Testing Information

Performed

Daily

Reported

3-5 days

Method

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry

Referral Lab

ARUP (80407)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

82384

Last Updated: Thursday, January 5, 2017 03:42:02 AM