CHROMOSOMAL ANALYSIS AMNIOTIC FLUID

Mnemonic

MISC

Synonym

Amniotic Fluid Chromosome Analysis - Karyotype - Karyotyping - Chromosomes - Chromosome Study - Cytogenetics

Specimen Collection Requirements

Collect

Amniotic Fluid, obtained using sterile technique

Handling

Express the first 1.0-2.0 mL into a separate tube to clear the specimen of possible maternal cells. Distribute the balance of the specimen evenly between two screw-top sterile polystyrene or polypropylene tubes. Specimens with gross blood should have 0.25 mL of sodium heparin (1,000 units/mL) aseptically added immediately to each tube. Note the addition of heparin on the tube. An additional 3.0-5.0 mL of specimen is needed if FISH testing for common aneuploidies is desired, refer to Prenatal Aneuploidy Screen. Keep specimen at ambient temperature and transport within 24 hours.

Preferred Volume

20.0 mL

Minimum Volume

All volumes are accepted

Transport

Room temperature within 24 hours of collection

Stability

Ambient: 24 hours; Refrigerated: Suboptimal, call Cytogenetics Lab at (503) 413-4613

Testing Information

Note

Clinical information including the indication for study must accompany the specimen. This includes the gestational age, ultrasound findings, prenatal screening results, previous reproductive history and any known family history of birth defects. Requisitions for cytogenetic studies are available upon request. Call Client Services for assistance with specimen transportation or to obtain supplies.

Performed

7 day/week

Reported

8-14 days

Method

Classical G-Band Chromosomal Analysis

Reference Values

Reference Values

Cytogenetic interpretation provided. A computer generated karyotype is also provided with each study.
Normal range:
46,XX, apparently normal female
46,XY, apparently normal male
If Genetic Counseling is needed, call (503) 413-5214.

Comments

For questions regarding the report, please call (503) 413-4613.

Last Updated: Thursday, January 5, 2017 03:41:58 AM