CRYPTOCOCCAL ANTIGEN DETECTION

Mnemonic

CCACSF for CSF CCAS for Serum

Synonym

Crypto-LA - India Ink Prep

Guidelines

This order is more appropriate and more sensitive than India Ink prep or fungal smear for the detection of Cryptococcus neoformans.

Specimen Collection Requirements

Patient Preparation

Decontaminate draw site with scrub/prep, use aseptic techniques.

Collect

Serum: One 5.0 mL gold (SST) or one 7.0 mL red top tube

CSF: One sterile, spill proof container

Handling

Place specimen in sterile container and refrigerate.

Preferred Volume

1.0 mL CSF or Serum (2.5 mL whole blood)

Minimum Volume

0.5 mL CSF or Serum (1.5 mL whole blood)

Transport

Refrigerated at 2-8°C

Rejection Criteria

Severely hemolyzed serum or any specimen collected in an anticoagulant

Stability

Ambient: 2 hours; Refrigerated: 72 hours; Frozen: Indefinite

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Immunochromatographic assay

Reference Values

Reference Values

Negative

Critical Values

Positive for Cryptococcus

Comments

Positive tests will be titered and the end point reported to a maximum of 1:2,048, unless further titration is specially requested.

CPT Codes

87327

Last Updated: Thursday, January 5, 2017 03:42:08 AM