CULTURE, BLOOD

Mnemonic

C

Synonym

BLOOD CULTURE

Guidelines

Two to three draws per initial 24 hour episode are recommended. For routine aerobic/anaerobic organisms, NOT for AFB, Fungus or special organisms, e.g. Bartonella, Legionella. This order can also be used for Bone Marrow.

Specimen Collection Requirements

Collect

Place 10 cc into an aerobic FA bottle (green) and 10 cc into an anaerobic bottle. For small children, a sample of 1 5 cc of blood is collected. The entire specimen is placed in the aerobic FA bottle.

Handling

Label each bottle with a computer label. Do not place the computer label over the bottle bar code tab or number. Include the time of the draw and your initials on the label. The phlebotomist should receive Blood cultures before delivering the blood culture to the Microbiology Department.

Preferred Volume

10.0 mL per bottle adults, 1.0-5.0 mL per pediatric bottle

Minimum Volume

1.0 mL

Transport

Room temperature, deliver ASAP

Rejection Criteria

Broken, leaking bottles

Stability

Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Testing Information

Performed

7 days/week

Method

Continuous Monitoring Instrumentation

Referral Lab

Samaritan Health Services

Reference Values

Reference Values

Negative

Critical Values

Positive stain or culture

CPT Codes

87040

Last Updated: Thursday, September 13, 2018 12:12:54 PM