CYTOMEGALOVIRUS (CMV) By PCR, BODY FLUID

Mnemonic

MISC

Specimen Collection Requirements

Collect

Body Fluid (pleural, peritoneal, ascites, pericardial, amniotic, or ocular), Urine, Respiratory Specimen: Sterile container
Spinal Fluid: Sterile vial(s)
Throat Swab: Culture transport swab
Tissue (brain, colon, kidney, liver, lung, etc): Sterile container containing 1-2 mL of sterile saline or multi-microbe medium (M5)
Bone marrow: EDTA

Handling

Body Fluid: Specimen grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by CMV DNA is not likely. Respiratory Specimens: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate are acceptable. Spinal Fluid: Do not centrifuge. Specimen grossly contaminated with blood may inhibit the PCR and produce false-negative results. The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by CMV DNA is not likely. Throat Swab: Place swab back into swab cylinder.

Preferred Volume

Urine: 2.0 mL

Minimum Volume

Body/Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL
Respiratory: 1 mL

Transport

Refrigerated

Rejection Criteria

Calcium alginate-tipped swab, wood swab, or transport swab containing gel; blood, serum, stool, paraffin blocks, or breast milk

Stability

Body Fluid, Spinal Fluid, Urine: Ambient: Unacceptable; Refrigerated: 7 days; Frozen: 7 days
Respiratory Specimen, Throat Swab, Tissue: Ambient: Unacceptable; Refrigerated: 7 days; Frozen: Unacceptable

Testing Information

Performed

Mon - Sat

Reported

2-3 days

Method

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Referral Lab

Mayo Medical Laboratory (LCMV)

Reference Values

Reference Values

Interpretation provided.

CPT Codes

87496

Last Updated: Thursday, January 5, 2017 03:42:07 AM