ELECTROPHORESIS, SERUM PROTEIN

Mnemonic

SPEP

Synonym

Serum Protein Electrophoresis - Serum Protein Electrophoresis with Reflex - PELP - PELP R - SPEP

Includes

Total Protein, Protein Electrophoresis, Pathologist Interpretation

If indicated: Specific Protein Identification by Immunofixation

Guidelines

Please indicate when ordering if Reflex to Immunofixation is required.

If indicated, Immunofixation will be reflexed for any pattern with a monoclonal spike, regardless of patient history.

If no indication is made, Immunofixation will not be reflexed.

Specimen Collection Requirements

Collect

Serum, One 5.0 mL gold (SST) or 7.0 mL red top tube. Fresh samples are recommended.

View image for Supply Item Number - 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum: SST - 30 minutes and red top tubes - 60 minutes). Centrifuge and separate serum from cells within 4 hours of collection. 

Refrigerate as soon as possible after collection.

Preferred Volume

3.0 mL

Minimum Volume

1.0 mL (2.5 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 4 days; Refrigerated (2-8°C): 2 weeks; Frozen (≤ -20°C): 6 months

Testing Information

Performed

Monday - Saturday

Reported

2-5 days

Method

Helena SPIFE 3000 Protein Electrophoresis and Immunofixation (if indicated), Beckman AU Colorimetry

Reference Values

Reference Values

Professional interpretation provided by Cascade Pathology Services.

CPT Codes

84155: Total Protein, Serum; 84165: Protein Electrophoresis, Serum; 86334: Immunofixation, Serum (if indicated)

Last Updated: Thursday, January 5, 2017 03:42:09 AM