ELECTROPHORESIS, URINE PROTEIN Mnemonic PEPU Synonym Urine Protein Electrophoresis - Urine Protein Electrophoresis with Reflex - U PELP R - U PELP RR - U PELP T - U PELP TR - UPEP Includes Total Protein, Protein Electrophoresis, Pathologist Interpretation If indicated: Specific Protein Identification by Immunofixation Guidelines Please indicate when ordering if Reflex to Immunofixation is required. If indicated, Immunofixation will be reflexed for any pattern with a monoclonal spike, regardless of patient history. If no indication is made, Immunofixation will not be reflexed. Specimen Collection Requirements Collect Preferred: Urine, 24-hour collection in sterile urine container with no preservatives. Refrigerate during collection. Also acceptable: Urine, random, first morning collection in a sterile container with no preservatives. Handling Keep sample refrigerated after collection. Record the total 24-hour collection volume on the order or requisition. Preferred Volume 100.0 mL Urine Minimum Volume 20.0 mL Urine Transport Refrigerated (2-8°C) Stability Room Temperature (18-26°C): 2 hours; Refrigerated (2-8°C): 72 hours; Frozen (≤ -20°C): 28 days Testing Information Performed Monday - Saturday Reported 2–5 days Method Helena SPIFE 3000 Protein Electrophoresis and Immunofixation (if indicated), Beckman AU Colorimetry Reference Values Reference Values Professional interpretation provided by Cascade Pathology Services. CPT Codes 84156: Total Protein, Urine; 84166: Protein Electrophoresis, Urine; 86335: Immunofixation, Urine (if indicated) Last Updated: Thursday, January 5, 2017 03:42:09 AM