ENTEROVIRUS DETECTION BY RT-PCR, PLASMA
Specimen Collection Requirements
Plasma or Serum, one 5.0 mL lavender (EDTA) or gold (SST) top tube
Centrifuge blood sample and remove plasma or serum before transporting.
Ambient: 8 hours; Refrigerated: 48 hours; Frozen: 3 months
Reverse Transcription/Polymerase Chain Rreaction
Negative: Enterovirus nucleic acid not detected by RT-PCR.
Positive: Enterovirus nucleic acid detected by RT-PCR.
A negative result does not rule out the presence of PCR inhibitors in the patient specimen or Enterovirus nucleic acid in concentrations below the level of detection of the assay.
Thursday, January 5, 2017 03:42:09 AM