EXTRACTABLE NUCLEAR ANTIGEN ANTIBODIES

Mnemonic

ENA

Synonym

Anti-ENA - Anti-RNP - Anti-Sm IgG - Anti-SSB - Antibodies to Extractable Nuclear Antigens - Autoantibodies to Scl 70 Antigen - Ribonucleoprotein Autoantibodies - Ro Ab - Scl 70 Antibodies - Smith (ENA) Antibody IgG - Smith Antigen - Smith Autoantibodies - SSB (La) (ENA) Antibody IgG - SSB LA

Includes

SCL70 AB
JO1 AB
SM AB
RNP AB
RO52 AB
RO60 AB
SSB AB
 

Specimen Collection Requirements

Collect

Serum, one gold (SST) or 7.0 mL red top tube.

Handling

Allow serum to clot completely at room temperature (minimum – SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

1.0 mL serum

Minimum Volume

0.5 mL serum (whole blood 1.2 mL minimum draw)

Transport

Frozen

Rejection Criteria

Plasma, microbially contaminated, heat treated or specimens containing visible particulates. Grossly hemolyzed, grossly lipemic or icteric serum should not be used.

Stability

Room Temp (18-26’C) 8 hours, Refrigerated (2-8’C) 48 hours, Frozen (<-20’C) 1 year

Testing Information

Note

This test can be ordered as a panel with all seven ENA’s or individually. 

Performed

Monday, Wednesday, Friday

Reported

3-5 days

Method

Chemiluminescent Immunoassay

Reference Values

Reference Values

SCL-70 Antibody:           Reference Value
<20 CU                              Negative
≥20 CU                              Positive

JO-1 Antibody:
<20 CU                              Negative
≥20 CU                              Positive

SM Antibody:  
<20 CU                              Negative
≥20 CU                              Positive          

RNP Antibody:    
<20 CU                              Negative
≥20 CU                              Positive

Ro52 Antibody:
<20 CU                              Negative
≥20 CU                              Positive

 

Ro60 Antibody:
<20 CU                              Negative
≥20 CU                              Positive

SSB Antibody:
<20 CU                              Negative
≥20 CU                              Positive

 
Reference values apply to all ages.

 

 

 

 

 

 

 

 

CPT Codes

86235 (X7)

Last Updated: Thursday, January 5, 2017 03:42:11 AM