FACTOR V MUTATION (LEIDEN) ANALYSIS Mnemonic FVMT Synonym Factor V - Leiden - Factor V Leiden - G1691A - c.1691G>A - 1691G>A - R506Q - F5 - c.1601G>A - p.Arg534Gln Specimen Collection Requirements Collect One lavender (EDTA) whole blood tube Handling Whole blood sample must be submitted in original container. Aliquot tubes will not be accepted. Do not separate whole blood. Preferred Volume 1.0 mL Minimum Volume 0.5 mL Transport Refrigerated (2-8°C) Rejection Criteria Specimens collected in heparin, samples with insufficient volume, specimens tested in non-molecular labs, aliquoted specimens, specimens past stability and clotted specimens. Stability Ambient: Up to 3 days; Refrigerated: Up to 7 days; Frozen: Up to 6 weeks. Testing Information Note Critical sterile. Samples cannot be shared with other laboratory departments unless molecular testing is performed first. Performed Mon - Fri, batched. Reported 6-8 days Method Real-time Polymerase Chain Reaction (PCR) using the cobas® Factor II and Factor V Test (IVD) on the cobas® z 480 analyzer Reference Values Reference Values Interpretation provided. Comments Though rare, mutations within the genomic DNA regions of the Factor V gene covered by the primers or probes used in the cobas® Factor II and Factor V Test may result in failure to detect the presence of the Factor V G1691A mutation. The presence of PCR inhibitors may cause invalid or incorrect results. CPT Codes 81241 Last Updated: Thursday, January 5, 2017 03:42:10 AM