FACTOR V MUTATION (LEIDEN) ANALYSIS

Mnemonic

FVMT

Synonym

Factor V - Leiden - Factor V Leiden - G1691A - c.1691G>A - 1691G>A - R506Q - F5 - c.1601G>A - p.Arg534Gln

Specimen Collection Requirements

Collect

One lavender (EDTA) whole blood tube

Handling

Whole blood sample must be submitted in original container. Aliquot tubes will not be accepted. Do not separate whole blood.

Preferred Volume

1.0 mL

Minimum Volume

0.5 mL

Transport

Refrigerated (2-8°C)

Rejection Criteria

Specimens collected in heparin, samples with insufficient volume, specimens tested in non-molecular labs, aliquoted specimens, specimens past stability and clotted specimens. 

Stability

Ambient: Up to 3 days; Refrigerated: Up to 7 days; Frozen: Up to 6 weeks.

Testing Information

Note

Critical sterile. Samples cannot be shared with other laboratory departments unless molecular testing is performed first.

Performed

Mon - Fri, batched.

Reported

6-8 days

Method

Real-time Polymerase Chain Reaction (PCR) using the cobas® Factor II and Factor V Test (IVD) on the cobas® z 480 analyzer

Reference Values

Reference Values

Interpretation provided.

Comments

Though rare, mutations within the genomic DNA regions of the Factor V gene covered by the primers or probes used in the cobas® Factor II and Factor V Test may result in failure to detect the presence of the Factor V G1691A mutation. The presence of PCR inhibitors may cause invalid or incorrect results.

CPT Codes

81241

Last Updated: Thursday, January 5, 2017 03:42:10 AM