LEUKOCYTE ALKALINE PHOSPHATASE

Mnemonic

LAPST

Synonym

LAP - LAP Stain – LAP Score- Leukocyte Alkaline Phosphatase

Specimen Collection Requirements

Collect

Green (sodium or lithium heparin)

Handling

Protect both smears and whole blood from light and pack accordingly to avoid breakage.

Transport 5 mL whole blood (Min: 1 mL) AND 6 unfixed, well-prepared smears (Min: 6 smears)

Preferred Volume

Blood: 5 mL dark green top (sodium heparin) or mint green top (lithium heparin) tube.

Slides: 6 unfixed well-made peripheral smears. Peripheral blood smears must be made from sodium or lithium heparin tube.

Minimum Volume

1 mL whole blood

6 unfixed, well-prepared smears (Min: 6 smears)

Transport

Room temperature.Send Sunday through Thursday only. Whole blood must be received within 24 hours of collection; smears must be made within 24 hours of collection and received within 7 days of collection.

Rejection Criteria

Specimens collected in EDTA. Poorly prepared smears (too thick or no feather edge). Broken or fixed smears. Specimens that have been spun. Smears made from anything other than heparin. Smears made from blood older than 24 hours. Whole blood not protected from light. Smears not protected from light.

Refrigerated: Unacceptable; Frozen: Unacceptable

Stability

Blood: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable

Unfixed Smears: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable

Testing Information

Performed

Monday – Friday

Reported

1-5 days

Method

Cytochemical Stain

Referral Lab

ARUP Laboratories (Test Code 0049000, LAP)

Reference Values

Reference Values

Female: 33-149 (no units)
Male: 22-124 (no units)

CPT Codes

85540

Last Updated: Thursday, January 5, 2017 03:42:19 AM