METHOTREXATE

Mnemonic

METHO

Synonym

Mexate® - Methopterin®

Guidelines

For information on when to collect specimen, please refer to Therapeutic Drug Monitoring (TDM) Guidelines for Pediatrics and Adults

Specimen Collection Requirements

Collect

Serum, 7.0 mL red top tube (NO GEL TUBES)

Handling

Allow serum to clot completely at room temperature (minimum of 60 minutes).
Centrifuge and separate serum from cells ASAP. PROTECT SPECIMEN FROM EXPOSURE TO LIGHT.

Preferred Volume

1.0 mL Serum

Minimum Volume

0.5 mL Serum (1.2 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Plasma, Gel Tubes, or hemolyzed specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 4 hours; Refrigerated (2-8°C): 2 weeks; Frozen (≤ -20°C): 28 days

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Homogenous Enzyme Immunoassay

Reference Values

Therapeutic Range

Toxic Levels

CPT Codes

80204

Last Updated: Thursday, January 5, 2017 03:42:21 AM