PARANEOPLASTIC SYNDROME ANTIBODY PANEL WITH REFLEX TO ANNA TITER, PCCA TITER AND NEURONAL IMMUNOBLOT

Mnemonic

MISC

Specimen Collection Requirements

Collect

Serum separator tube

Handling

Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to a Standard Transport Tube. (Min: 0.75 mL)

Preferred Volume

2.0 mL

Minimum Volume

0.75 mL

Transport

Frozen

Rejection Criteria

Contaminated, heat-inactivated, hemolyzed, or lipemic specimens.

Stability

After separation from cells: Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 1 year

Testing Information

Note

ANNA antibodies are screened by IFA. If the IFA screen is positive at 1:10, then a titer and immunoblot (Hu and Ri) will be added. If Purkinje cell screen is positive at 1:10, then a titer and immunoblot (Yo) will be added. Additional charges apply.

Method

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot

Referral Lab

ARUP 0051226

Reference Values

Reference Values

Number Components Reference Interval Neuronal Nuclear Antibody (ANNA) IgG Screen, by IFA < 1:10 0050892 Neuronal Nuclear Antibody (ANNA) Reflex IFA Titer, IgG < 1:10 No antibody detected 0059444 Purkinje Cell Cytoplasmic Antibody by IFA with Reflex to Titer & Immunoblot Components Reference Interval Purkinje Cell Cytoplasmic Ab (PCCA or PCA-1) Screen Less than 1:10 Purkinje Cell Cytoplasmic Ab (PCCA or PCA-1) Titer < 1:10 Purkinje Cell Cytoplasmic Ab (PCCA or PCA-1) Immunoblot Negative Purkinje Cell Antibody, Titer < 1:10 Neuronal Nuclear Antibody IgG, Immunoblot Anti-Hu Negative Neuronal Nuclear Antibody IgG, Immunoblot Anti-Ri Negative 0051091 Neuronal Antibody IgG, Immunoblot Anti-Yo Negative

CPT Codes

86255 ANNA; if reflexed, add 86256 ANNA titer and 83516 Immunoblot; 86255 Purkinje cell screen; if reflexed, add 86256 Purkinje titer

Last Updated: Thursday, January 5, 2017 03:42:21 AM