PML-RARA TRANSLOCATION, t(15;17) BY RT-PCR, QUANTITATIVE

Mnemonic

<b><span style='color:red'><u>PMLQNT</span></u></b>

Synonym

t(15;17)

Guidelines

The t(15;17) chromosomal translocation which leads to the PML/RARα fusion transcript is strongly associated with acute promyelocytic leukemia (APL). The detection of this highly characteristic abnormality is useful in the accurate diagnosis of APL. These tumor-specific markers can also be used to monitor the remission status of affected patients. RT-PCR provides a rapid alternative to cytogenetic analysis and may identify PML/RARα gene rearrangement in the absence of detectable t(15;17) by the latter technique.

Specimen Collection Requirements

Collect

Whole Blood or Bone Marrow, one 5.0 mL lavender (EDTA) or 3.0 mL bone marrow

Handling

Samples must be received within 48 hours of collection due to lability of RNA.

Preferred Volume

5.0 mL whole blood or 3.0 mL bone marrow (EDTA)

Minimum Volume

1.0 mL whole blood or bone marrow (EDTA)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Serum or plasma, frozen or clotted whole blood or bone marrow, specimens collected in preservatives other than EDTA,severely hemolyzed specimens

Stability

Ambient: 1 hour; Refrigerated: 2 days; Frozen: Unacceptable

Testing Information

Performed

RNA isolation: 7 days/week; Assay: Varies

Method

Polymerase Chain Reaction

Referral Lab

ARUP (56100)

Reference Values

Reference Values

Negative

CPT Codes

83891, 83902, 83913, 83898 x2, 83896 x4, 83912

Last Updated: Thursday, January 5, 2017 03:42:22 AM