PYRUVIC ACID Mnemonic PYR Synonym Pyruvate Specimen Collection Requirements Patient Preparation Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet. Collect Whole Blood, one green (sodium or lithium heparin) top tube Handling REQUIRES SPECIAL HANDLING. Immediately after blood is drawn, add exactly 1.0 mL whole blood to a chilled pyruvate collection tube containing 2.0 mL 8% (w/v) perchloric acid. Collection tubes are available from the Legacy Referral Department. Mix well for 30 seconds and place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1,500 x g. Decant supernatant into a transport tube and freeze. Preferred Volume 2.0 mL Minimum Volume 1.0 mL Transport Frozen Rejection Criteria If less than 1.0 mL of blood is added to the collection tube, pH of the supernatant will be too low for testing to be performed. Stability Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks Testing Information Performed 7 days/week Reported 1-2 days Method Enzymatic Referral Lab ARUP (80310) Reference Values Reference Values 0.030-0.107 mmol/L (venous blood) CPT Codes 84210 Last Updated: Thursday, January 5, 2017 03:42:28 AM