VITAMIN D, 25-HYDROXY LEVEL

Mnemonic

VITD

Synonym

Vitamin D - 25-Hydroxyvitamin D2 - 25-Hydroxyvitamin D3 - Calciferol, 25-Hydroxycalciferol - Cholecalciferol - Ergocalciferol

Includes

Total 25-Hydroxyvitamin, 25-Hydroxyvitamin D2, and 25-Hydroxyvitamin D3

Guidelines

Samples on Infants or Children < 1 year old: These samples will be sent to Legacy Laboratory for testing.

Specimen Collection Requirements

Collect

Site-specific: Consult Samaritan Epic Catalog or contact your local laboratory.

Handling

Allow serum to clot completely at room temperature (minimum: 30 minutes). Centrifuge and separate serum from cells ASAP. Freeze serum.

Preferred Volume

1.0 mL

Minimum Volume

0.5 mL

Transport

Frozen (at or below -20°C)

Stability

AFTER SEPARATION FROM CELLS: Ambient (20-25°C): 8 hours, Refrigerated (2-8°C): 4 days; Frozen (at or below -20°C): 24 weeks

Testing Information

Performed

Daily as received

Method

Colorimetry

Referral Lab

Samaritan Health Services Laboratories

Reference Values

Reference Values

Reportable Range:	5-120 ng/mL
Deficient:	</= 20 ng/mL
Insufficient:	21-29 ng/mL
Sufficient:	>/= 30 ng/mL
Possible Toxicity:	> 100 ng/mL

Comments

Visible hemolysis may cause interference. Samples should be redrawn if possible. Samples with moderate hemolysis (>200 mg/dL) will have falsely elevated results. Grossly lipemic samples are to be airfuged prior to testing.

CPT Codes

82306

Last Updated: Thursday, January 17, 2019 09:20:34 AM