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ACTH Stimulation Cortisol 30 Minutes

Mnemonic

CORTSTIM30

Synonym

CORTISOL - CORT - ACTH - ACTH STIM - CORTISOL STIMULATION

Guidelines

Part of assessing ACTH Cortisol Stimulation at 0, 30, 60 Minutes

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

Supply Item Number - 101235 or 101192

Handling

Allow serum to clot completely at room temperature (minimum – SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

1.0 mL Serum

Minimum Volume

0.3 mL Serum (0.8 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Grossly hemolyzed or plasma specimens

Stability

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 48 hours; Frozen (≤ -20°C): 2 months

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Chemiluminescent Immunoassay

Reference Values

Reference Values

Greater than 7 ug/dL increase

Peak greater than 20 ug/mL

CPT Codes

82533

Last Updated: Thursday, February 11, 2021 06:51:00 PM


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