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Anal-Rectal Pap

Mnemonic

PATH GYN REQUEST

Synonym

Anal Cytology

Specimen Collection Requirements

Collect

  1. A Dacron swab, FLOQ swab or cytobrush, if tolerated by the patient, are the best collection devices for anal cytology.
  2. If using the Dacron swab, moisten the swab with water. Do not use lubricant. If using the FLOQ swab, moistening the swab with water is not necessary. Do not use lubricant.
  3. The Dacron swab, FLOQ swab or cytobrush should be inserted approximately 1.5 to 2 inches into the anal canal. It is important to use either the Dacron or FLOQ and not a cotton swab, as cells tend to cling to cotton and do not release easily into cytology collection fluids.
  4. Once inserted deep enough into the anus (necessary in order to collect both rectal columnar and anal squamous cells) the swab or brush should be pulled out, applying some pressure to the wall of the anus, rotating the swab or brush in a spiral motion along the way.
  5. Immediately after collection, all devices employed should be vigorously rinsed into a single vial of cytology fixative (SurePath or ThinPrep).
  6. Record two patient identifiers on the vial. Also record the patient information and medical history on the requisition form.
  7. Place the vial and requisition in a specimen bag for transport to the laboratory.

Handling

All containers must be labeled with the patient’s first and last name and a second identifier, such as date of birth. Liquid based Pap fixative vials should be submitted with the caps securely tightened to avoid leakage.

Preferred Volume

SurePath: 10.0 mL, ThinPrep: 20.0 mL

Minimum Volume

SurePath: 10.0 mL, ThinPrep: 20.0 mL

Transport

Room temperature or refrigerated

Rejection Criteria

Unlabeled, expired or frozen specimens will be rejected.

Stability

4 weeks from collection at room temperature or refrigerated.

Testing Information

Performed

Mon - Fri

Reported

2-3 days

Method

Pap stain/microscopy

Referral Lab

Professional interpretation provided by Cascade Pathology. 

Last Updated: Tuesday, January 5, 2021 06:16:38 PM


Client Services: 503-413-1234