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Anti-Nuclear Antibody ELISA Screen Reflex Cascade

Mnemonic

ANA R-CASCADE

Synonym

ANA R-CASCADE

Includes

Anti-Nuclear Antibody (ANA) ELISA Screen with reflex to IFA Titer and Pattern. Positive IFA will reflex to DSDNA AB and ENA PAN (SCL-70 AB, JO-1 AB, SM AB, RNP AB, RO52 AB, RO60 AB, SSB AB).

Guidelines

ANA specimens are screened using an ELISA assay. All specimens that do not screen negative are confirmed using Hep-2 cells, and if possible, the titer and pattern will be reported. Positive IFA will reflex to DSDNA AB and ENA PAN. 

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) top tube

Supply Item Number - 101235

Handling

Allow blood to clot for 30 minutes. Centrifuge and separate serum from cells immediately. Pour serum into plastic tube.

Preferred Volume

2.0 mL serum

Minimum Volume

1.0 mL serum (whole blood 2.5 mL minimum draw)

Transport

Refrigerated if arrival in the Legacy Central Laboratory is within 48 hours. Freeze if transport requires longer than 48 hours.

Rejection Criteria

Plasma and other body fluids; severely lipemic or bacterially contaminated specimens

Stability

COLLECTION TO INITIATION OF TESTING AFTER SEPARATION FROM CELLS: Ambient: 8 hours; Refrigerated (2-8°C): 48 hours; Frozen (at or below -20°C): 21 days

Testing Information

Performed

Mon - Fri

Reported

ANA R-CASCADE SCREEN:

24-72 hours

 

ENA PAN & DSDNA AB:

3-5 days after reflexing from positive ANA R-CASCADE

Method

ANA Screen-Enzyme-linked Immunosorbent Assay (ELISA), ANA IFA-Indirect Fluorescent Antibody, ENA PAN-Chemiluminescent Immunoassays, DSDNA AB-Chemiluminescent Immunoassays.

Reference Values

Reference Values

Negative (ANA ELISA); see individual analytes

CPT Codes

86038 ANA: if reflexed, add 86039 ANA Titer, 86225 DSDNA AB, 86235 x7 ENA PAN

Last Updated: Tuesday, August 27, 2019 06:54:53 PM


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